Kutty Selva Nandakumar, Ph.D., Dr.Med.Sci.

Assistant Professor                                                               

Medical Inflammation Research, MBB, Karolinska Institute

Scheeles väg 2 B2 Plan 4, 171 77 Stockholm                                     

Tel: 08-524   87715; 08-524   86337; Fax: 08-524 87750: e-mail: Nandakumar.Kutty-Selva@ki.se

 

Education

 

Lund University, Medical Inflammation Research, Medical Faculty – Lund, Sweden. (http://www.med.lu.se/) Mentor: Prof. Rikard Holmdahl

Doctor of Philosophy (Ph.D.), Immunogenetics – January 2006

 

Madurai Kamaraj University, Department of Immunology, School of Biological Sciences – Madurai, India. (http://www.mkuniversity.org) Mentor: Prof. VR. Muthukkaruppan

Doctor of Philosophy (Ph.D.), Immunology – January 1996

 

University of Madras – Department of Biochemistry, PostGraduate Institute of Basic Medical Sciences, Taramani Campus, Chennai, India. (http://www.unom.ac.in)

Master of Science (M.Sc.), Biochemistry – April 1988

 

University of Madras – Department of Chemistry, Sacred Heart College, Tiruppattur, (http://www.shctpt.edu/)

Bachelor of Science (B.Sc.), Chemistry – April 1986

 

Lab and Research Experience

 

 

 

 

 

 

 

 

Commission of Trust

 

 

 

 

Field of Expertise

 

Immunology - Immunogenetics – inflammation – Arthritis - Infectious diseases (Salmonellosis) - Tumor immunology - Hybridoma – Antibodies - Animal models of human diseases – Testing vaccines in mice – in vivo experiments

 
Teacher Training course

Basic course on Oral communication (2002), Lund University; Basic Course in Education for University Teachers (2009), LIME, Karolinska Institute; Postgraduate supervisor training course (2009), LIME, Karolinska Institute;  Advanced course on Communication and learning (2009), LIME, Karolinska Institute.


Teaching experience (Total hours: 51.30 h+ 40 h)

 

Teaching in animal models for human diseases, immunology and genetics for biomedical students, organic chemistry for undergraduate students and research supervision.

 

Research training for short duration (min period: 3 months during 2002-2009)

 

M.Sc. students: 3; Ph.D. students: 3; M.D. students: 2; BMA student: 1


Faculty Opponent


Ph.D. dissertation - Mohammed Mullazehi (May 2009). “Anti-collagen type II autoantibodies in an acute phenotype of early Rheumatoid arthritis”. Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Uppsala, Sweden. 

 

Project Grants (year 2007) - Total: 415.000 SEK

 

1. Title: "Genetic and Immunologic characterization of antibody-mediated arthritis in mice" and "Identification of genes controlling both inflammation and metabolic syndrome using a heterogeneous stock"

 

Foundations: Alfred Österlunds Foundation, Stiftelsen Professor Nanna Svartz fond, Reumatikerforbundet, Konung Gustaf V:s 80-Årsfond and Lund University Diabetic centre (LUDC)

 

Project Grants (year 2008) –Total: 800.480 SEK

 

Title: Studies on genes and pathogenesis of inflammatory diseases

 

Foundations: Tore Nilssons fond, Alfred Österlunds Foundation, Greta och Johan Kocks Stiftelser, Stiftelsen Professor Nanna Svartz fond, Reumatikerforbundet, Alex och Eva Wallströms stiftelse and Konung Gustaf V:s 80-Årsfond

 

Project Grants (year 2009)

 

1. Individual Project Grants (year 2009) – Total: 925.000 SEK (so far)

 

1. Title: Studies on genes and pathogenesis of inflammatory diseases

 

Foundations: Konung Gustaf V:s 80-Årsfond, Åke Wibergs stiftelse and Professor Nanna Svartz fond, Reumatikerforbundet, Alex and Eva Wallstroms fond, AnneGreta Holger Crafoord fond and KI fond (Fobi 2009).                         

 

2. Swedish National Collaborative Project Grant (year 2009 - 2011)

 

Title: "Towards immune specific diagnosis and prevention of rheumatoid arthritis"

 

(Main applicant: Rikard Holmdahl; Co-applicants: Johan Backlund, Vivi Malmstrom, Kutty Selva Nandakumar and Ronald Van Vollenhoven)

 

VR Collaborative Grant                                     6.000.000 SEK

 

3. International Collaborative project Grant (years 2009 – 2011)

 

Title: "Analysis of Host Response to Modified Biocompatible Stimuli-responsive Polymers and Autoantigens Using Murine Experimental arthritis"

 

Main applicants: Kutty Selva Nandakumar (Sweden) and Ashok Kumar (India)

 

VR Link Grant                                                   600.000 SEK

 

Patents/applications: 3

 

1. Björck L, Holmdahl R and Nandakumar KS – "IdeS for treatment of IgG antibody mediated diseases and conditions in the areas of autoimmunity, hematology and transplantation" (2005). Patent Number: WO2006131347: Publication date:

2006-12-14.

 

2. Olsson A, Collin M, Björck L, Shannon O, Holmdahl R and Nandakumar KS –  "Therapeutic application of Endo S" (2006).

 

3. Blom A, Holmdahl R and Nandakumar KS - "C4bp polypeptides and arthritis" (2008).

 

Current Supervisor Responsibility

 

Dorota Klaczkowska: Main Supervisor  - Ph.D. student in Lund University and Karolinska Institutet from Poland HT 2009 - current

Ia Khamzade: Main Supervisor - Ph.D. student in Karolinka Institutet from Gerogia HT 2009 – current

 

Ingrid Lindh: Co-Supervisor - Ph.D. student in Karolinska Institutet from Sweden – HT 2008 – current

 

Publications

 

Kielland A, Blom T, Nandakumar KS,  Holmdahl R, Blomhoff R and Carlsen H (2009). In vivo imaging of reactive oxidative species during inflammation using the luminescent probe L-012. Free Radic Biol Med 2009 June 16 [Epub ahead of print]  PubMed

Nandakumar KS (2009). Pathogenic antibody recognition of cartilage. Cell and Tissue Res Jun 9. [Epub ahead of print] PubMed

Johansson ÅCM, Nandakumar KS, Persson AM, Olsson I, Hansson M (2009). Targeting of human soluble TNF-a receptor into secretory lysosomes of murine hematopoietic cells in vivo as a principle for immunoregulation in inflammation and malignancy Exp Hematol May 29. [Epub ahead of print] PubMed

Uysal H, Bockermann R, Nandakumar KS, Sehnart B, Bajtner E, Engström Å, Burkhardt H, Thunnissen M and Holmdahl R (2009). Structure and pathogenicity of antibodies specific for citrullinated type II collagen. J Exp Med 206: 449-62. PubMed

Hultqvist M, Nandakumar KS, Björklund U and Holmdahl R (2009). The novel small molecule Rob 803 is effective in reducing severity of disease in the mouse models of autoimmune disorders. Ann Rheum Dis 68:130-5. Epub 2008 Mar 17 PubMed

Blom AM , Nandakumar KS , Holmdahl R (2009). C4 binding protein (C4BP) inhibits development of experimental arthritis Ann Rheum Dis 68:136-42. Epub 2008 Feb 14.  Co-first authors. PubMed

Geng H, Carlsen S, Nandakumar KS, Holmdahl R, Aspberg A, Oldberg Å and Mattsson R (2008). The role of cartilage matrix protein (COMP) in collagen II induced arthritis Arthritis Res Ther 10:R134. PubMed

Nilsang S, Nehru V, Plieva F, Nandakumar KS, Rakshit SK, Holmdahl R, Mattiasson B and Kumar A (2008). Three-dimensional culture for monoclonal antibody production by hybridoma cells immobilized in macroporous gel particles. Biotechnology Progress. 24:1122-31.

Nandakumar KS and Holmdahl R (2008). Role of anti-hinge antibodies in therapeutic cleavage of IgG: response to Drs. Lutz and Fumia. Trends in Immunology. 29:355-6. Epub 2008 Jul 2 PubMed

Rowley M, Nandakumar KS and Holmdahl R (2008). The role of collagen antibodies in mediating arthritis. Mod Rheumatology Jun 3. [Epub ahead of print].PubMed

Carlsen S, Nandakumar KS, Backlund J, Holmberg J, Hultqvist M, Vestberg M, and Holmdahl R (2008). Cartilage oligomeric matrix protein induces chronic arthritis in mice. Arthritis and Rheumatism. 2008 Jul;58(7):2000-11. PubMed

Nandakumar KS* and Holmdahl R (2008). Threapeutic cleavage of IgG – new avenues for treating inflammation. Trends Immunol 29:173-8. Epub 2008 Mar 6. PubMed

Uysal H, Sehnert B, Nandakumar KS, Böiers U, Burkhardt H, Holmdahl R, Thunnissen M (2008). The crystal structure of the pathogenic collagen type II specific mouse monoclonal antibody CIIC1 Fab: Structure to function analysis. Mol Immunol 45:2196-2204. Epub 2008 Feb 1. PubMed

Nandakumar KS*, Bajtner E, Hill L, Böhm B, Rowley MJ, Burkhardt H and Holmdahl R (2008).Arthritogenic antibodies specific for a major type II collagen triple helical epitope bind and destabilize cartilage independent of inflammation. Arthritis Rheum 58:184-196. * - corresponding author. PubMed

Nandakumar KS and Holmdahl R (2007). Collagen antibody induced arthritis. Methods Mol Med 136:215-23. PubMed

Nandakumar KS*, Collin M, Olsen A, Nimmerjahn F, Blom A, Ravetch JV and Holmdahl R (2007). Endoglycosidase treatment abrogates IgG arthritogenicity – importance of IgG glycosylation in arthritis. Eur J Immunol 37:2973-82. * - corresponding author.PubMed

Gelderman KA, Hultqvist M, Pizzolla A, Zhao M, Nandakumar KS, Mattsson R and Holmdahl R (2007). Macrophages suppress T cell responses by producing reactive oxygen species (ROS). J Clin Invest 117:3020-8. PubMed

Nandakumar KS*, Johansson BP, Björck L and holmdahl R (2007). Blocking arthritis by cleavage of IgG antibodies in vivo. Arthritis Rheum 56:3253-60. * - corresponding author.PubMed

Nilsang S, Nandakumar KS, Galaev IY, Rakshit SK, Holmdahl R, Mattiasson B and Kumar A (2007). Monoclonal antibody production using a new supermacroporous cryogel perfusion reactor. Biotechnology Progress 23:932-939. PubMed

Jonsson, IM, Verdrengh M, Brisslert M, Lindblad S, Bokarewa M, Islander U, Carlsten H, Ohlsson C, Nandakumar KS, Holmdahl R, Tarkowski A (2007). Ethanol prevents development of destructive arthritis. PNAS 104:258-263. PubMed

Madsen T, Ujvari B, Nandakumar KS, Hasselquist D and Holmdal R. (2007). Do infectious prey select for high levels of natural antibodies in tropical pythons? Evol Ecol 21:271–279. WOS

Nandakumar KS* and Holmdahl R (2006). Antibody induced arthritis – disease mechanisms and genes involved at the effector phase of arthritis. Arthritis Res Ther 8:223-233. Review. * - corresponding author.PubMed

Lindqvist A-K B, Johannesson M, Johansson ÅCM, Nandakumar KS, Blom A, Holmdahl R (2006). Backcross and partial advanced intercross analysis of NOD gene mediated effects on collagen induced arthritis reveals an interactive effect by two major loci J Immunol 177:3952-3959. PubMed

Carlsen S, Nandakumar KS and Holmdahl R (2006). Type IX collagen deficiency enhances binding of cartilage specific antibodies and arthritis severity Arthritis Res Ther 8:R102. PubMed

Sehnert B, Gierer P, Ibrahim S, Kuhl A, Voll R, Nandakumar KS, Holmdahl R, Hallmann R, Vollmar B, and Burkhardt H (2006). Modulation of granulocyte-endothelial interactions by antileukoproteinase: inhibition of anti-type II collagen antibody-induced leukocyte-attachment to the synovial endothelium. Arthritis Res Ther 8:R95. PubMed

Nandakumar KS* and Holmdahl R (2006). Arthritis induced with cartilage-specific antibodies is IL-4 dependent. Eur J Immunol 36:1608-1618. (Cover figure). * - corresponding author. PubMed

Dzhambazov B, Nandakumar KS, Kihlberg J, Fugger L, Holmdahl R and Vestberg M. (2006). Therapeutic vaccination of active arthritis with a glycosylated collagen type II peptide in complex with MHC class II molecules. J Immunol 176:1525-33. PubMed

Kumar A, Bansal V, Nandakumar KS, Galaev IY, Roychoudhury PK, Holmdahl R, Mattiasson B (2006). Bioprocess integration for the production and isolation of urokinase from animal cell culture using supermacroporous cryogel matrices Biotechnol Bioeng 93:636-46. PubMed

Treschow A, Teige I, Nandakumar KS, Holmdahl R and Issazadeh-Navikas S (2005). Stromal cells and osteoclasts are responsible for an exacerbated collagen-induced arthritis in IFN-b deficient mice. Arthritis and Rheum 52: 3739-40. PubMed

Bajtner E, Nandakumar KS, Engström Å and Holmdahl R (2005). B cell fine specificity response to triple helical type II collagen along the disease course of chronic relapsing collagen induced arthritis. Arthritis Res Ther 7:R1148-R1157. PubMed

Nandakumar KS* and Holmdahl R (2005). Efficient induction of collagen antibody induced arthritis (CAIA) using four antibodies specific for the major epitopes recognized in both collagen-induced arthritis and rheumatoid arthritis. J Immunol methods 304:126-136. * - corresponding author.PubMed

Crombie DE, Turer M, Biurrun B, Wood B, McNaughton D, Nandakumar KS, Holmdahl R, Van Damme M, Rowley MJ (2005).  Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro. Arthritis Res Ther 7:R927-R937. PubMed

Amirahmadi SF, Whittingham S, Crombie D, Nandakumar KS, Holmdahl R, Mackay IR, Van Damme MP, Rowley MJ (2005). Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells. Arthritis and Rheum 52:1897-1906. PubMed

Nandakumar KS* and Holmdahl R (2005). A genetic contamination in MHC congenic mouse strains reveals a locus on chromosome 10 which determine autoimmunity and arthritis susceptibility. Eur J Immunol 35:1275-82. * - corresponding author.PubMed

Johannesson M, Karlsson J, Wernhoff P, Nandakumar KS, Lindqvist A-K, Olsson L, Cook A, Andersson A, Holmdahl R (2005). Dissection of a mouse QTL associated with arthritis; formulation of the phenotype and the genetic interactions through a partial advanced intercross strategy. Genes and immunity 6:175-185. PubMed

Li J, Ny A, Leonardsson G, Nandakumar KS, Holmdahl R and Ny T (2005). The plasminogen activator/plasmin system is essential for development of the joint inflammatory phase of collagen type II-induced arthritis. Am J pathol 166:783-92.  PubMed

Kumar A, Rodr’guez-Caballero A, Plieva FM, Galaev IY, Nandakumar KS, Kamihira M, Holmdahl R, Orfao A, Mattiasson B (2005). Affinity Binding of Cells to Cryogel Adsorbents with Immobilized Specific Ligands: Effect of Ligand Coupling and Matrix Architecture. J Molecular Recognition 18:84-93. PubMed

Lange F, Bajtner E, Rintisch C, Nandakumar KS, Sack U, Holmdahl R (2005). Methotrexate ameliorates T cell dependent autoimmune arthritis and encephalomyelitis but not antibody or fibroblast induced arthritis. Ann Rheum Dis 64:599-605. PubMed

Nandakumar KS*, Backlund J, Vestberg M and Holmdahl R (2004). Collagen type II specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII reactive T cells. Arthritis Res Ther 6:R544-50. * - corresponding author.PubMed

Xu Y, Ramsland PA, Davies JM, Scealy M, Nandakumar KS, Holmdahl R, Rowley MJ (2004).Two monoclonal antibodies to precisely the same epitope of type II collagen select non-crossreactive phage clones by phage display: implications for autoimmunity and molecular mimicry. Molecular Immunol 41:411-419. PubMed

Sehnert B, Cavcic A, Böhm B, Kalden JR,Nandakumar KS, Holmdahl R, Burkhardt H (2004). Antileukoproteinase: Modulation of neutrophil function and therapeutic effects on anti-collagen type II-antibody-induced arthritis. Arthritis Rheum 50:2347-59. PubMed

Hietala MA, Nandakumar KS, Persson L, Fahlen S, Holmdahl R and Pekna M (2004). Complement activation by both alternative and classical pathways is critical for the effector phase of arthritis. Eur J Immunol 34:1208-16. PubMed

Hansson AS, Johannesson M, Svensson L, Nandakumar KS, Heineg Œrd H and Holmdahl R (2004). Relapsing Polychondritis, induced in Mice with Matrilin 1, is an Antibody- and Complement-Dependent Disease. Am J Pathol 164: 959-66. PubMed

Nandakumar KS*, Svensson L, Holmdahl R (2003). Collagen type II specific monoclonal antibody induced arthritis (CAIA) in mice. Description of the disease and the influence of age, sex, and genes.  Am J pathol 163:1827-37. * - corresponding author.PubMed

Backlund J, Nandakumar KS, Bockermann R, Mori L Holmdahl R (2003). Genetic control of tolerance to type II collagen and development of arthritis in an autologous collagen-induced arthritis model. J Immunol 171:3493-9. PubMed

Nandakumar KS , Andren M , Martinsson P, Bajtner E, Hellstrom S, Holmdahl R, Kleinau  S (2003). Induction of arthritis by single monoclonal IgG anti-collagen type II antibodies and enhancement of arthritis in mice lacking inhibitory FcgRIIB.  Eur J Immunol 33:2269-77.  Co-first authors. PubMed

Burkhardt H, Koller T, Engstrom A, Nandakumar KS, Turnay J, Kraetsch HG, Kalden JR, Holmdahl R (2002). Antibody epitope specific recognition of collagen type II in rheumatoid arthritis is shared with arthritogenic antibodies in collagen induced arthritis. Arthritis Rheum 46:2339-48. PubMed

Svensson L, Nandakumar KS, Johansson AC, Jansson L, Holmdahl R (2002). IL-4 deficient mice develop less acute but more chronic relapsing arthritis. Eur J Immunol 32:2944-53. PubMed

Lindqvist AK, Bockermann R, Johansson AC, Nandakumar KS, Johannesson M, Holmdahl R (2002). Mouse models for rheumatoid arthritis. Trends in Genetics 18(6): S7-13. Review.

Johansson AC, Hansson AS, Nandakumar KS, Backlund J, Holmdahl R (2001). IL-10 deficient B10.Q mice develop more severe collagen induced arthritis but are protected from arthritis induced with anti-type II collagen antibodies. J Immunol 167: 3505–12. PubMed

Corthay A, Nandakumar KS, Holmdahl R (2001). Evaluation of the percentage of peripheral T cells with two different T cell receptor a chains and of their potential roles in autoimmunity. J Autoimmun 16:423-9. PubMed

Nandakumar KS*, Muthukkaruppan VR (1999). Influence of immunopotentiators on the anti-porin IgG subclass distribution and protective immunity against murine salmonellosis.  Scand J Immunol 50:188-94. * - corresponding author.PubMed

Nandakumar KS, Lakshmi Rao K, Pardhasaradhi BV, Khar A (1999). Upregulation of anti-tumor immunity by IL-12 gene transfected AK-5 tumor cells in vivo. Cytokines Cell Mol Ther 5:7-14. PubMed

Nandakumar KS, Varalakshmi C, Khar A (1998). Role of cytokines in the maintenance of the memory immune response induced by a rat histiocytoma in syngeneic hosts. Cytokines Cell Mol Ther 4:169-75. PubMed

Nandakumar KS, Kumari AL, Khar A (1997). Early expression of IL-12 p40 and IFN-g inhibits AK-5 tumor regression. Cytokines Cell Mol Ther 3:225-32. PubMed

Nandakumar KS, Palanivel V, Muthukkaruppan VR (1993). Diagnosis of Typhoid Fever: Detection of S. typhi porins specific antibodies by Inhibition ELISA. Clin Exp Immunol 94:317-21. PubMed

Muthukkaruppan VR, Nandakumar KS, Palanivel V (1992). Monoclonal antibodies against Salmonella porins: Generation and Characterization. Immunol Lett 33: 201-6. PubMed